AFAP1 Antibody

• Description :

  Actin filament-associated protein 1 is a protein that in humans is encoded by the AFAP1 gene. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q8N556

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A human recombinant partial protein (amino acids A498-T730) was used as the immunogen for the AFAP1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, FACS, Direct ELISA

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the AFAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This AFAP1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the AFAP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the AFAP1 antibody should be determined by the researcher.

• Image Legend :

  IHC staining of FFPE human lung cancer with AFAP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.