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PROM1 Antibody / CD133

CAT: 0800-RQ6236Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6236Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O43490
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A human recombinant partial protein (amino acids P531-H865) was used as the immunogen for the PROM1 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
8B6
Applications
WB, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PROM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PROM1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PROM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PROM1 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human Caco-2 cells with PROM1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PROM1 antibody.

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