CHD2 Antibody

• Description:

  Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays; and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  O14647

• Host:

  Rabbit

• Reactivity:

  Human, Monkey

• Immunogen:

  Amino acids DKLGEYKQPLPPLHPAVSDPRS from the human protein were used as the immunogen for the CHD2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CHD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CHD2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CHD2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CHD2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human SiHa cells with CHD2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CHD2 antibody.