DIO1 Antibody

• Description:

  Type I iodothyronine deiodinase is a protein that in humans is encoded by the DIO1 gene. It is mapped to 1p32.3. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P49895

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids K137-S249) was used as the immunogen for the DIO1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, IF, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the DIO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This DIO1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the DIO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the DIO1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human A549 cells with DIO1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.