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DIO1 Antibody

CAT: 0800-RQ5971Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5971Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Type I iodothyronine deiodinase is a protein that in humans is encoded by the DIO1 gene. It is mapped to 1p32.3. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P49895
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids K137-S249) was used as the immunogen for the DIO1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the DIO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DIO1 antibody is available for research use only.
Storage Conditions
After reconstitution, the DIO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DIO1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human A549 cells with DIO1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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