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Bid Antibody

CAT: 0800-RQ5945Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5945Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL
UniProt
P55957
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein (amino acids M1-VD195) was used as the immunogen for the Bid antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
10F4
Applications
WB, IHC-P, FACS, IF
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Bid antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Bid antibody is available for research use only.
Storage Conditions
After reconstitution, the Bid antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Bid antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with Bid antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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