Products for Research Use Only

WNT5B Antibody

CAT: 0800-RQ5929Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5929Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9H1J7
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids Q18-K218) was used as the immunogen for the WNT5B antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the WNT5B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This WNT5B antibody is available for research use only.
Storage Conditions
After reconstitution, the WNT5B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the WNT5B antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human U-87 MG cells with WNT5B antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= WNT5B antibody.
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