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ALAS1 Antibody

CAT: 0800-RQ5867Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5867Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P13196
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids M1-Q244) was used as the immunogen for the ALAS1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the ALAS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ALAS1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ALAS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ALAS1 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Flow cytometry testing of human HEL cells with ALAS1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ALAS1 antibody.

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