CYP2C19 Antibody

• Description :

  Cytochrome P450 2C19 (abbreviated CYP2C19) encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  P33261

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids QNQQSEFTIENLVITAADLLGAGTETT from the human protein were used as the immunogen for the CYP2C19 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  10G5

• Applications :

  WB, IHC-P, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the CYP2C19 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This CYP2C19 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the CYP2C19 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the CYP2C19 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  IHC staining of FFPE human liver cancer with CYP2C19 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.