MECP2 Antibody

• Description:

  MECP2 (methyl CpG binding protein 2) is a gene that encodes the protein MECP2. It is mapped to Xq28. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P51608

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids K119-R453) was used as the immunogen for the MECP2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  2G3

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the MECP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MECP2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MECP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the MECP2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) U-87 MG, 2) HeLa, 3) K562, 4) HEK293 and 5) PC-3 lysate with MECP2 antibody. Expected molecular weight: ~55 kDa (unmodified) and ~75 kDa (modified) .