GATA2 Antibody

• Description:

  GATA2 (GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al. (1997) mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al. (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al. (2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P23769

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids D23-S288) was used as the immunogen for the GATA2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, IF, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the GATA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This GATA2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the GATA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the GATA2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human A431 cells with GATA2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.