Products for Research Use Only
RegisterLogin

AKAP12 Antibody

CAT: 0800-RQ5698Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-RQ5698Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
A-kinase anchor protein 12, also called AKAP250, is an enzyme that in humans is encoded by the AKAP12 gene. The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9WTQ5
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Recombinant mouse protein (amino acids E387-H1620) was used as the immunogen for the AKAP12 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the AKAP12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This AKAP12 antibody is available for research use only.
Storage Conditions
After reconstitution, the AKAP12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the AKAP12 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with AKAP12 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q9WTQ5

A-kinase anchor protein 12

AKA12_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9WTQ5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Akap12
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
AKAP-12
EC number
—
Processing
—
Secondary accessions
Q80SS4, Q810D4, Q8BPK4, Q99MP1
Protein keywords

Coding sequence diversity

Alternative splicing

Molecular function

Calmodulin-binding

Cellular component

CytoplasmCytoskeletonMembrane

PTM

Isopeptide bondLipoproteinMyristatePhosphoproteinUbl conjugation

Technical term

Reference proteome

Domain

Repeat

Related Products

CatalogName
RQ6599AKAP 95 Antibody / AKAP8
R35661-100UGAKAP10 Antibody
F53944-0.05MLAKAP13 Antibody
F53944-0.2MLAKAP13 Antibody
R33163-100UGAKAP10 Antibody
R34745-100UGAKAP6 Antibody
R36240-100UGAKAP3 Antibody
R35909-100UGAKAP9 Antibody
R33594-100UGAKAP8 Antibody
RQ8698AKAP8 Antibody / A-kinase anchor protein 8 / AKAP 95

Alternative Products

CatalogName
70R-12998AKAP12 antibody

Popular Products