USP16 Antibody

• Description :

  Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene. It is mapped to 21q21.3. This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q9Y5T5

• Host :

  Rabbit

• Reactivity :

  Human, Rat, Monkey

• Immunogen :

  Recombinant human protein (amino acids K9-L823) was used as the immunogen for the USP16 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IF, FACS, Direct ELISA

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the USP16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This USP16 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the USP16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the USP16 antibody should be determined by the researcher.

• Image Legend :

  IHC staining of FFPE human lung cancer with USP16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.