RegisterLogin

PCNA Antibody

CAT: 800-RQ5594Size: 100 µgDry Ice: NoHazardous: No
100 µgSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase delta in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry (intracellular) : 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P12004
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids M1-S261) was used as the immunogen for the PCNA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PCNA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
After reconstitution, the PCNA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PCNA antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with PCNA antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.

Related Products

CatalogName
V4803SAF-100UGProliferating Cell Nuclear Antigen Antibody / PCNA
V4803-20UGProliferating Cell Nuclear Antigen Antibody / PCNA
V4803-100UGProliferating Cell Nuclear Antigen Antibody / PCNA
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.05MLAntibody
F53954-0.2MLAntibody
F53952-0.05MLAntibody
F53952-0.2MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody

Alternative Products

CatalogName
MBS8580893-01PCNA Antibody
MBS856728-01PCNA Antibody
MBS8515984-01PCNA Antibody
MBS8586425-01PCNA Antibody
MBS7122267-01PCNA Antibody
MBS8587057-01PCNA Antibody
MBS9602827-01PCNA Antibody
MBS9524541-01PCNA Antibody
MBS9215348-01PCNA Antibody
MBS9215349-01PCNA Antibody

Popular Products