TNNT3 Antibody

• Description:

  Fast skeletal muscle troponin T (fTnT) is a protein that in humans is encoded by the TNNT3 gene. It is mapped to 11p15.5. The binding of Ca (2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca (2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca (2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca (2+) -mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B) .

• Specifications:

  Western blot: 0.25-0.5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P45378

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A human recombinant protein (amino acids A45-K251) was used as the immunogen for the TNNT3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the TNNT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TNNT3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TNNT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the TNNT3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) rat skeletal muscle and 2) mouse skeletal muscle with TNNT3 antibody. Predicted molecular weight: ~32 kDa, routinely observed at 35~38 kDa.