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PRKCD Antibody

CAT: 0800-RQ5529Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5529Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC) . It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. It is also a part of a noncanonical WNT signaling cascade.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q05655
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids F4-D676) was used as the immunogen for the PRKCD antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the PRKCD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PRKCD antibody is available for research use only.
Storage Conditions
After reconstitution, the PRKCD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PRKCD antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear, plasma membrane
Image Legend
Western blot testing of human 1) placenta, 2) ThP-1, 3) Caco-2 and 4) HEK293 lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.

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