PRKCD Antibody

• Description :

  Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC) . It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. It is also a part of a noncanonical WNT signaling cascade.

• Specifications :

  Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q05655

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A human recombinant protein (amino acids F4-D676) was used as the immunogen for the PRKCD antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IF FACS, Direct ELISA

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the PRKCD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This PRKCD antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the PRKCD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the PRKCD antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, nuclear, plasma membrane

• Image Legend :

  Western blot testing of human 1) placenta, 2) ThP-1, 3) Caco-2 and 4) HEK293 lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.