Desmin Antibody

• Description :

  DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What�s more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  P17661

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A human recombinant protein (amino acids M1-T304) was used as the immunogen for the Desmin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b

• Clone :

  2B5

• Applications :

  WB, IHC-P, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the Desmin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Desmin antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Desmin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the Desmin antibody should be determined by the researcher.

• Location :

  Cytoplasmic, membranous

• Image Legend :

  IHC staining of FFPE human skeletal muscle with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.