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Hsp105 Antibody

CAT: 0800-RQ5517Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5517Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q92598
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant protein (amino acids Y653-D858) was used as the immunogen for the Hsp105 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3D10
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Hsp105 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Hsp105 antibody is available for research use only.
Storage Conditions
After reconstitution, the Hsp105 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Hsp105 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human intestinal cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Product Datasheet
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