Products for Research Use Only
RegisterLogin

BAK1 Antibody

CAT: 0800-RQ5500Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-RQ5500Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry/Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q16611
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids A22-S211) was used as the immunogen for the BAK1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
4C2
Applications
WB, IHC-P, ICC/IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the BAK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This BAK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the BAK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the BAK1 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with BAK1 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Related Products

CatalogName
R32246BAK Antibody / BAK1
R30507BAK Antibody
F42652-0.4MLBak Antibody
F42652-0.08MLBak Antibody
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody

Alternative Products

CatalogName
70R-13976BAK1 antibody

Popular Products