EpCAM Antibody

• Description:

  The EPCAM gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [RefSeq]

• Specifications:

  Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50

• UniProt:

  P16422

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide specific to human EpCAM was used as the immunogen for the EpCAM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  CIB-5

• Type:

  Recombinant

• Applications:

  WB, IHC-P, IF

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the EpCAM antibody at -20oC.

• Limitations:

  This EpCAM antibody is available for research use only.

• Storage Conditions:

  Store the EpCAM antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the EpCAM antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  IHC staining of FFPE mouse small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.