EpCAM Antibody

• Description:

  May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation.

• Specifications:

  Western blot: 1:1000, Immunoprecipitation: suitable

• UniProt:

  P16422

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein was used as the immunogen for this EpCAM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  1D5-B6-B10

• Applications:

  WB, IP

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

• Reconstitution:

  Store the EpCAM antibody at -20oC.

• Limitations:

  This EpCAM antibody is available for research use only.

• Storage Conditions:

  Store the EpCAM antibody at -20°C.

• Formulation:

  In PBS with 50% glycerol and 0.03% ProClin 300

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the EpCAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of lysate from CHO-K1, EpCAM transfected CHO-K1, MCF7 (+ control), HCT116 (+ control), and HeLa (- control) cell lysate using EpCAM antibody at 1:1000. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated) .