PCNA Antibody

• Description:

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. [RefSeq]

• Specifications:

  Western blot: 1:3000-1:10000, Immunohistochemistry (FFPE) : 1:50-1:200

• UniProt:

  P12004

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide specific to human PCNA was used as the immunogen for the PCNA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  DO-16

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the PCNA antibody at -20oC.

• Limitations:

  This PCNA antibody is available for research use only.

• Storage Conditions:

  Store the PCNA antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the PCNA antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nuclear

• Image Legend:

  Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.