PCNA Antibody

CAT: 0800-RQ5380Size: 100 µLDry Ice: NoHazardous: No
CAT#:0800-RQ5380Size:100 µL
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AVAILABILITY: InStock
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Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. [RefSeq]
CAS Number
9000-83-3
Specifications
Western blot: 1:3000-1:10000, Immunohistochemistry (FFPE) : 1:50-1:200
UniProt
P12004
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A synthetic peptide specific to human PCNA was used as the immunogen for the PCNA antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
DO-16
Type
Recombinant
Applications
IHC-P, WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the PCNA antibody at -20oC.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Store the PCNA antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the PCNA antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

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