PCNA Antibody

• Description :

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. [RefSeq]

• Specifications :

  Western blot: 1:3000-1:10000, Immunohistochemistry (FFPE) : 1:50-1:200

• UniProt :

  P12004

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A synthetic peptide specific to human PCNA was used as the immunogen for the PCNA antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG

• Clone :

  DO-16

• Type :

  Recombinant

• Applications :

  IHC-P, WB

• Purity :

  Affinity purified

• Format :

  Purified

• Buffer :

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution :

  Store the PCNA antibody at -20oC.

• Limitations :

  This PCNA antibody is available for research use only.

• Storage Conditions :

  Store the PCNA antibody at -20°C.

• Formulation :

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes :

  Optimal dilution of the PCNA antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Nuclear

• Image Legend :

  Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.