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HERC5 Antibody

CAT: 0800-RQ5045Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5045Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9UII4
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids F793-G1024 from the human protein were used as the immunogen for the HERC5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the HERC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HERC5 antibody is available for research use only.
Storage Conditions
After reconstitution, the HERC5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HERC5 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Immunofluorescent staining of FFPE human A431 cells with HERC5 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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