ER alpha Antibody

• Description:

  Estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. [RefSeq]

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100, Immunofluorescence: 1:50-1:100

• UniProt:

  P03372

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide specific to human ER alpha / ESR1 was used as the immunogen for the ER alpha antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  IE-5

• Type:

  Recombinant

• Applications:

  IHC-P, WB, IF

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the ER alpha antibody at -20oC.

• Limitations:

  This ER alpha antibody is available for research use only.

• Storage Conditions:

  Store the ER alpha antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the ER alpha antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human 1) MCF7 and 2) T-47D cell lysate with ER alpha antibody. Predicted molecular weight ~66 kDa.