Products for Research Use Only
RegisterLogin

SRCIN1 Antibody

CAT: 0800-RQ4950Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-RQ4950Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322) . Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. Indirect immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-3ug/10^6 cells, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9C0H9
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids E189-E287 from the human protein were used as the immunogen for the SRCIN1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the SRCIN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SRCIN1 antibody is available for research use only.
Storage Conditions
After reconstitution, the SRCIN1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SRCIN1 antibody should be determined by the researcher.
Location
Cytoplasm, plasma membrane
Image Legend
Western blot testing of 1) human T-47D, 2) human MDA-MB-453, 3) rat brain and 4) mouse brain lysate with SRCIN1 antibody at 0.5ug/ml. Expected molecular weight: 130-140 kDa.

Related Products

CatalogName
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody
V8674-100UGInhibin alpha Antibody / INHA antibody
R20161SAF-250UGLambda Light Chain Antibody / Lambda Antibody
R30971B-Actin Antibody / Beta Actin Antibody

Alternative Products

CatalogName
A14967-50UGSRCIN1 Antibody
MBS1751511-01Anti-SRCIN1 Antibody
OASG05478-100ULSRCIN1 Antibody - middle region
A08110-1Anti-SRCIN1 Antibody Picoband®
GTR17691531SRCIN1 Rabbit Polyclonal Antibody
A08110Anti-p140Cap SRCIN1 Antibody
GTR18556196SRCIN1 Rabbit Polyclonal Antibody (Biotin)
GTR18748301SRCIN1 Rabbit Polyclonal Antibody (Fluoro647)
OAAF04583-100UG-BiotinSRCIN1 Antibody : Biotin (OAAF04583-Biotin)
GTR18556194SRCIN1 Rabbit Polyclonal Antibody (FITC)

Popular Products