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DARS2 Antibody

CAT: 0800-RQ4930Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ4930Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL) . The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q6PI48
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids D334-A448 from the human protein were used as the immunogen for the DARS2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF/ICC, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the DARS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DARS2 antibody is available for research use only.
Storage Conditions
After reconstitution, the DARS2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DARS2 antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) A431, 2) K562, 3) A549, 4) PC-3, 5) U-2 OS, 6) Caco-2 and 7) HEK293 lysate with DARS2 antibody at 0.5ug/ml. Predicted molecular weight ~74 kDa.

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