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APRT Antibody

CAT: 0800-RQ4891Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4891Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP) . It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08030
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids RAEVVECVSLVELTSLKGRERL from the mouse protein were used as the immunogen for the APRT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the APRT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This APRT antibody is available for research use only.
Storage Conditions
After reconstitution, the APRT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the APRT antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) T-47D, 2) HEK293, 3) K562 and 4) Caco-2 lysate with APRT antibody at 0.5ug/ml. Predicted molecular weight ~20 kDa.

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