PRC1 Antibody

• Description:

  Protein regulator of cytokinesis 1 (PRC1) is a key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression. [UniProt]

• Specifications:

  Western blot: 1:500-1:2000

• UniProt:

  O43663

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human PRC1 was used as the immunogen for the PRC1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  EH-16

• Type:

  Recombinant

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the PRC1 antibody at -20oC.

• Limitations:

  This PRC1 antibody is available for research use only.

• Storage Conditions:

  Store the PRC1 antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the PRC1 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human HeLa cell lysate with PRC1 antibody. Expected molecular weight ~70-80 kDa, may be observed as a doublet.