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CRISPR-Cas9 Antibody

CAT: 0800-RQ4684Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-RQ4684Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) . CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA) . Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. [UniProt]
CAS Number
9000-83-3
Specifications
Western blot: 1:1000-1:5000
UniProt
J7RUA5
Host
Rabbit
Reactivity
Staphylococcus aureus
Immunogen
A recombinant protein fragment derived from Staphylococcus aureus was used as the immunogen for the CRISPR-Cas9 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
ABCH-3
Type
Recombinant
Applications
WB
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the CRISPR-Cas9 antibody at -20oC.
Limitations
This CRISPR-Cas9 antibody is available for research use only.
Storage Conditions
Store the CRISPR-Cas9 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the CRISPR-Cas9 antibody should be determined by the researcher.
Image Legend
Western blot testing of CRISPR-Cas9 expression in 1) human 293T cell lysate transfected with CRISPR-Cas9, 2) human HEK293, 3) mouse NIH3T3 cell lysate and 4) rat PC12 cell lysate with CRISPR-Cas9 antibody.

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