NOVA1 Antibody

• Description :

  RNA-binding protein Nova-1 is a protein that in humans is encoded by the NOVA1 gene. It is mapped to 14q12. This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein)

• UniProt :

  P51513

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids T416-V509 from the human protein were used as the immunogen for the NOVA1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, IF, FACS, Direct ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the NOVA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This NOVA1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the NOVA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the NOVA1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC staining of FFPE mouse brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.