CACNA2D2 Antibody

• Description:

  Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2 gene. This gene is mapped to 3p21.31. It encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

• Specifications:

  Western blot: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

• UniProt:

  Q9NY47

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids Q66-Q284 from the human protein were used as the immunogen for the CACNA2D2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CACNA2D2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CACNA2D2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CACNA2D2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CACNA2D2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human MCF7 cell lysate with CACNA2D2 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.