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APE1 Antibody

CAT: 0800-RQ4515Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4515Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P27695
Host
Mouse
Reactivity
Human
Immunogen
Human APE1 recombinant protein (amino acids P2-L318) was used as the immunogen for the APE1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
5C11
Applications
IHC-P, WB
Purity
Protein G affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the APE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This APE1 antibody is available for research use only.
Storage Conditions
After reconstitution, the APE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the APE1 antibody should be determined by the researcher.
Location
Nucleus, cytoplasm, ER, mitochondria
Image Legend
IHC testing of FFPE human intestinal cancer with APE1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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