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Cytochrome C Antibody

CAT: 0800-RQ4499Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4499Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL
UniProt
P99999
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Human Cytochrome C recombinant protein (amino acids G2-E105) was used as the immunogen for the Cytochrome C antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
5F10
Applications
WB, IHC-P, FACS, IF
Purity
Protein G affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Cytochrome C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cytochrome C antibody is available for research use only.
Storage Conditions
After reconstitution, the Cytochrome C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Cytochrome C antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Flow cytometry testing of human K562 cells with Cytochrome C antibody at 1ug/10^6 cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.

UniProtKB · P99999

Cytochrome c

CYC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P99999
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CYCS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4D166, B2R4I1, P00001, Q6NUR2, Q6NX69, Q96BV4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

ApoptosisElectron transportRespiratory chainTransport

Disease

Disease variant

Ligand

HemeIronMetal-binding

Cellular component

Mitochondrion

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