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COBRA1 Antibody

CAT: 0800-RQ4476Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4476Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cofactor of BRCA1, also known as COBRA1, is a human gene that encodes NELF-B. NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2), either NELF-C or NELF-D (TH1L), and NELF-E (RDBP) . NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II. COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)
UniProt
Q8WX92
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human COBRA1 recombinant protein (amino acids M1-E252) was used as the immunogen for the COBRA1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the COBRA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This COBRA1 antibody is available for research use only.
Storage Conditions
After reconstitution, the COBRA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the COBRA1 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Western blot testing of 1) rat kidney, 2) mouse kidney and 3) human MDA-MB-231 lysate with COBRA1 antibody at 0.5ug/ml. Predicted molecular weight ~66 kDa.
Product Datasheet
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