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Leptin Antibody

CAT: 0800-RQ4442Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4442Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, ELISA (Capture of mouse recombinant protein) : 1-5 µg/mL
UniProt
P41160
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Mouse Leptin recombinant protein (amino acids V22-C167) was used as the immunogen for the Leptin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Leptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Leptin antibody is available for research use only.
Storage Conditions
After reconstitution, the Leptin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Leptin antibody should be determined by the researcher.
Location
Secreted
Image Legend
IHC testing of FFPE mouse lung tissue with Leptin antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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