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P65 Antibody

CAT: 0800-RQ4439Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4439Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein that in humans is encoded by the RELA gene. It is mapped to 11q13.1. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)
UniProt
Q04206
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human NF-kB p65 recombinant protein (amino acids F99-S551) was used as the immunogen for the p65 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the p65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This p65 antibody is available for research use only.
Storage Conditions
After reconstitution, the p65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the p65 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Western blot testing of human 1) COLO-320, 2) A549, 3) HepG2, 4) MDA-MB-231, 5) PANC-1 and 6) A375 lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

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