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IRE1 Antibody

CAT: 0800-RQ4356Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4356Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 (IRE1 or IRE1a) is an enzyme that in humans is encoded by the ERN1 gene. This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR) . The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O75460
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids R158-L280 was used as the immunogen for the IRE1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the IRE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IRE1 antibody is available for research use only.
Storage Conditions
After reconstitution, the IRE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the IRE1 antibody should be determined by the researcher.
Location
Cytoplasm (Endoplasmic reticulum membrane)
Image Legend
Western blot testing of 1) human A549, 2 ((h) SK-OV-3, 3) (h) PANC-1, 4) rat brain and 5) mouse brain lysate with IRE1 antibody at 0.5ug/ml. Predicted molecular weight ~110 kDa, observed here at ~170 kDa.

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