Neurexin 1 Antibody

• Description:

  Neurexin-1-alpha is a protein that in humans is encoded by the NRXN1 gene. This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca (2+) -dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia.

• Specifications:

  Western blot: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P58400

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant human protein corresponding to amino acids D141-E294 was used as the immunogen for the Neurexin 1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Neurexin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Neurexin 1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Neurexin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Neurexin 1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane

• Image Legend:

  Western blot testing of human 1) U-87 MG and 2) SHG-4 cell lysate with Neurexin antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.