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USP7 Antibody / HAUSP

CAT: 0800-RQ4283Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4283Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin-specific-processing protease 7 (USP7), also known as ubiquitin carboxyl-terminal hydrolase 7 or herpesvirus-associated ubiquitin-specific protease (HAUSP), is an enzyme that in humans is encoded by the USP7 gene. The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q93009
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids L258-D483 was used as the immunogen for the USP7 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF/ICC, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the USP7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This USP7 antibody is available for research use only.
Storage Conditions
After reconstitution, the USP7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the USP7 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human 1) HeLa, 2) 22RV1, 3) A431, 4) HepG2, 5) HepG2, 6) SGC-7901, 7) A549, 8) rat brain and 9) mouse testis lysate with USP7 antibody at 0.5ug/ml. Predicted molecular weight ~128 kDa, observed at ~135 kDa.
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