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CD80 Antibody

CAT: 0800-RQ4248Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4248Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) . CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL, Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P33681
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids V35-L244 was used as the immunogen for the CD80 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, WB, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CD80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD80 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD80 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CD80 antibody should be determined by the researcher.
Location
Membrane
Image Legend
Immunohistochemical staining of FFPE mouse spleen tissue with CD80 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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