CD80 Antibody

• Description:

  Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) . CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.

• Specifications:

  Immunohistochemistry (FFPE) : 2-5 µg/mL, Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P33681

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant human protein corresponding to amino acids V35-L244 was used as the immunogen for the CD80 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  IHC-P, WB, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the CD80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD80 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD80 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CD80 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Membrane

• Image Legend:

  Immunohistochemical staining of FFPE mouse spleen tissue with CD80 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.