Products for Research Use Only
RegisterLogin

MDC1 Antibody

CAT: 0800-RQ4235Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-RQ4235Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q14676
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human protein corresponding to amino acids A534-Q626 was used as the immunogen for the MDC1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, IF, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the MDC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MDC1 antibody is available for research use only.
Storage Conditions
After reconstitution, the MDC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MDC1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC testing of FFPE human testis tissue with MDC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Related Products

CatalogName
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.05MLAntibody
F53954-0.2MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody
V8674-100UGInhibin alpha Antibody / INHA antibody
R20161SAF-250UGLambda Light Chain Antibody / Lambda Antibody
R20179SAF-250UGLambda Light Chain Antibody / Lambda Antibody

Alternative Products

CatalogName
GWB-5CF5D7MDC1 Antibody

Popular Products