MDC1 Antibody

• Description :

  Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q14676

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A recombinant human protein corresponding to amino acids A534-Q626 was used as the immunogen for the MDC1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  IHC-P, IF, FACS, ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the MDC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This MDC1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the MDC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the MDC1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC testing of FFPE human testis tissue with MDC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.