ICOS Antibody

• Description:

  Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

• Specifications:

  IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, FACS: 1-3ug/10^6 cells

• UniProt:

  Q9Y6W8

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant human protein corresponding to amino acids E21-K140 was used as the immunogen for the ICOS antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  FACS, IHC-P, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the ICOS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This ICOS antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the ICOS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the ICOS antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell membrane

• Image Legend:

  IHC testing of FFPE human tonsil tissue with ICOS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.