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RNF186 Antibody

CAT: 0800-RQ4154Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4154Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1. This gene is mapped to chromosome 1p36.13 based on an alignment of theRNF186 sequence with the genomic sequence (GRCh38) . Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1. Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9NXI6
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human partial protein corresponding to amino acids R86-L159 was used as the immunogen for the RNF186 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the RNF186 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RNF186 antibody is available for research use only.
Storage Conditions
After reconstitution, the RNF186 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RNF186 antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) human SW620 and 2) human HepG2 cell lysate with RNF186 antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
Product Datasheet
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