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Pf4 Antibody / Platelet factor 4

CAT: 0800-RQ4050Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4050Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P06765
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
A recombinant rat partial protein corresponding to amino acids V30-S105 was used as the immunogen for the Pf4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Pf4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Pf4 antibody is available for research use only.
Storage Conditions
After reconstitution, the Pf4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Pf4 antibody should be determined by the researcher.
Location
Secreted
Image Legend
Western blot testing of rat spleen lysate with Pf4 antibody at 0.5ug/ml. Expected molecular weight: ~8/16/32 kDa (monomer/dimer/tetramer) .

UniProtKB · P06765

Platelet factor 4

PLF4_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P06765
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pf4
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
PF-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Chemotaxis

Molecular function

CytokineHeparin-binding

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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