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Ada Antibody

CAT: 0800-RQ4049Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4049Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Adenosine Deaminase (also known as Adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, ELISA (Capture) : 0.1-0.5 µg/mL
UniProt
Q920P6
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
A recombinant rat partial protein corresponding to amino acids A2-H241 was used as the immunogen for the Ada antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Ada antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ada antibody is available for research use only.
Storage Conditions
After reconstitution, the Ada antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Ada antibody should be determined by the researcher.
Image Legend
IHC testing of FFPE mouse intestine tissue with Ada antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

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