ERCC1 Antibody

• Description :

  DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

• CAS Number :

  9007-83-4

• Specifications :

  Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  P07992

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A recombinant human partial protein corresponding to amino acids E203-V296 was used as the immunogen for the ERCC1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the ERCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This ERCC1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the ERCC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the ERCC1 antibody should be determined by the researcher.

• Image Legend :

  Western blot testing of 1) rat stomach and 2) mouse stomach lysate with ERCC1 antibody at 0.5ug/ml. Predicted molecular weight: 25-36 kDa (multiple isoforms) .