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Tbp Antibody

CAT: 0800-RQ4014Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4014Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TBP (TATA-binding protein) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P29037
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
A recombinant mouse partial protein corresponding to amino acids L38-A107 was used as the immunogen for the Tbp antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Tbp antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Tbp antibody is available for research use only.
Storage Conditions
After reconstitution, the Tbp antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Tbp antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) rat testis and 2) rat ovary tissue lysate with Tbp antibody at 0.5ug/ml. Predicted molecular weight ~38 kDa.

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