Prothrombin Antibody

• Description:

  F2 (Coagulation Factor II), also known as prothrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1) . Thrombin also inhibits coagulation by activating protein C.

• Specifications:

  Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P00734

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein corresponding to amino acids Y67-R124 was used as the immunogen for the Prothrombin antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Prothrombin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Prothrombin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Prothrombin antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted

• Image Legend:

  IHC testing of FFPE human colon cancer tissue with Prothrombin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.