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Prothrombin Antibody

CAT: 0800-R32993Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32993Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
F2 (Coagulation Factor II), also known as prothrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1) . Thrombin also inhibits coagulation by activating protein C.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P00734
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human partial protein corresponding to amino acids Y67-R124 was used as the immunogen for the Prothrombin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Prothrombin antibody is available for research use only.
Storage Conditions
After reconstitution, the Prothrombin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Prothrombin antibody should be determined by the researcher.
Location
Secreted
Image Legend
IHC testing of FFPE human colon cancer tissue with Prothrombin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

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