TRFP Antibody / MED20

• Description:

  MED20, also called TRFP or TRF-proximal protein, is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.

• Specifications:

  Western Blot: 0.5-1 µg/mL, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)

• UniProt:

  Q9H944

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant human protein corresponding to amino acids M1-R212 was used as the immunogen for the TRFP antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, Direct ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the TRFP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TRFP antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TRFP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the TRFP antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) rat brain and 2) human PANC-1 lysate with TRFP antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.