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Beta Catenin Antibody

CAT: 0800-R32807Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32807Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry: 2-4 µg/mL, Immunofluorescence: 5 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL (BSA-free formulation available)
UniProt
P35222
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids A2-K233 was used as the immunogen for the Beta Catenin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, ICC, FACS, Direct ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose, 0.025% sodium azide
Reconstitution
After reconstitution, the beta Catenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Beta Catenin antibody is available for research use only.
Storage Conditions
After reconstitution, the Beta Catenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Beta Catenin antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of 1) human placenta, 2) human A431, 3) human SK-OV-3, 4) rat heart and 5) mouse testis lysate with Beta Catenin antibody at 0.5ug/ml. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

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