Choline acetyltransferase Antibody

• Description:

  Choline acetyltransferase (also called ChAT or CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL

• UniProt:

  P28329

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Amino acids D446-R652 from the human protein were used as the immunogen for the Choline acetyltransferase antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Choline acetyltransferase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Choline acetyltransferase antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Choline acetyltransferase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Choline acetyltransferase antibody should be determined by the researcher.

• Prediction Reactivity:

  Human

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  Western blot testing of 1) rat testis and 2) mouse testis tissue with Choline acetyltransferase antibody at 0.5ug/ml. Predicted molecular weight ~83/74/70 kDa (isoforms M/S/R), observed here at ~84 kDa.